檀碧波,李勇,赵群,范立侨,狄岩,温转.MiR-514与胃癌细胞凋亡的关系及机制[J].转化医学杂志,2019,8(2):80-84
MiR-514与胃癌细胞凋亡的关系及机制
Relationship between miR-514 and apoptosis of gastric cancer and related mechanism
  
DOI:
中文关键词:  胃癌  微小RNA 514  凋亡  病理参数
英文关键词:Gastric cancer  MicroRNA-514 (miR-514)  Apoptosis  Clinicopathological parameters
基金项目:河北省自然科学基金面上项目(H2016206173);河北省科技支撑项目(14277779D);河北省卫生和计划生育委员会重点科技研究计划(20160176)
作者单位
檀碧波 河北医科大学第四医院外三科 
李勇 河北医科大学第四医院外三科 
赵群 河北医科大学第四医院外三科 
范立侨 河北医科大学第四医院外三科 
狄岩 河北省医学情报研究所 
温转 河北医科大学第四医院质量控制办公室 
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中文摘要:
      目的 探讨微小RNA-514(microRNA-514,miR-514)与胃癌细胞凋亡的关系及机制。方法 实时定量PCR检测80例胃癌组织及癌旁正常胃黏膜组织中miR-514的表达情况,分析miR-514与患者各病理参数之间的关系。应用miR-514模拟物转染人胃癌细胞株MGC803,检测转染前后细胞凋亡率及凋亡相关基因的情况。结果 胃癌组织中miR-514水平(0.3619±0.2103)明显低于癌旁正常胃黏膜(0.6943±0.3032)(t=-8.0573;P<0.001);miR-514表达与肿瘤的淋巴结转移有关(t=-2.5620,P=0.012 3);miR-514模拟物转染人胃癌细胞株MGC803后细胞的活性明显降低而凋亡率明显增高(P<0.05);转染后凋亡相关基因Bcl-2、xIAP的mRNA和蛋白表达明显降低,而Bax的mRNA和蛋白表达明显增高(P<0.05)。结论 胃癌组织中miR-514的表达减弱,miR-514可能通过调节一些凋亡基因表达而参与肿瘤的凋亡过程。
英文摘要:
      Objective To investigate the relationship between microRNA-514 (miR-514) and apoptosis of gastric cancer and related mechanism. Methods Expression of miR-514 in gastric cancer tissues and cancer-adjacent normal tissues from 80 patients were detected with real-time PCR, and relationship between miR-514 and clinicopathological parameters was investigated. miR-514 mimics was utilized to transfected into gastric cancer cell line MGC803, then variation of apoptosis rate and related genes was detected. Results Level of miR-514 was lower in gastric cancer tissues (0.3619±0.2103) than that in cancer-adjacent normal tissues (0.6943±0.3032) (t=-8.0573, P<0.001). Expression of miR-514 was related with lymph node metastasis (t=-2.5620, P=0.0123). After transfection of miR-514 mimics, cell activity was decreased, while apoptotic rate of MGC803 increased significantly(P<0.05); mRNA and protein of Bcl-2, xIAP were decreased significantly, while Bax increased significantly (P<0.05). Conclusion Reduction of miR-514 existed in gastric cancer tissues, which may be invovled in apoptosis of gastric cancer cells by regulating apoptosis related genes.
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