朱文娅,常艳.蛋氨酸-胆碱缺乏饮食诱导非酒精性单纯性脂肪肝小鼠模型建立及动态观察[J].转化医学杂志,2020,9(4):219-222
蛋氨酸-胆碱缺乏饮食诱导非酒精性单纯性脂肪肝小鼠模型建立及动态观察
Mice model of non-alcoholic simple fatty liver building with methionine-choline-deficient diet and dynamic observation
  
DOI:
中文关键词:  非酒精性脂肪性肝病  非酒精性单纯性脂肪肝  蛋氨酸-胆碱缺乏饮食  动物模型
英文关键词:Non-alcoholic fatty liver disease (NAFLD)  Non-alcoholic simple fatty liver (NAFL)  Methionine-choline-deficient diet (MCDD)  Animal model
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作者单位
朱文娅 中国人民解放军总医院第六医学中心干部消化内科 
常艳 中国人民解放军总医院第六医学中心干部消化内科 
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中文摘要:
      目的非酒精性单纯性脂肪肝(non-alcoholic simple fatty liver,NAFL)作为非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)的早期阶段,发病隐匿、症状不典型,建立合适的动物模型对于研究NAFL的规律、改善诊断和治疗手段的意义重大。方法24只小鼠随机分为观察组(n=12)和对照组(n=12);观察组小鼠给予蛋氨酸-胆碱缺乏饮食(methionine-choline-deficient diet,MCDD)饲养3周,对照组给予正常饮食;动态观察小鼠的一般情况、按期称取体重;分别于第8、15、22天各处死4只观察组小鼠和对照组小鼠,后采集血清标本检测丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、甘油三酯(triglycerides,TG),肝脏标本处理后进行病理学诊断并进行评分量化。结果观察组小鼠一般情况、体重均有异于正常对照组,从第8天起小鼠即出现肝脂肪性变,而第8、15天小鼠的肝脏病理非酒精性脂肪性肝病活动度评分(non-alcoholic fatty liver disease active score,NAS)均在3~4分之间,与第22天比较差异具有统计学意义(t=2.601,P=0.04;t=3.57,P=0.019);血清中ALT、AST水平随造模时间的延长呈上升趋势,但各时间节点与对照组差异比较无统计学意义;TG的水平也随时间变化呈现增长趋势,且各时间节点均与对照组比较差异具有统计学意义,ALT水平与病理评分关系中等(r=0.574,P=0.032)。结论MCDD诱导2周可形成稳定的NAFL动物模型,该动物模型出现显著的一般情况改变、体重下降、血脂升高。
英文摘要:
      ObjectiveNon-alcoholic simple fatty liver (NAFL) is early stage of non-alcoholic fatty liver disease (NAFLD), with no typical symptom, therefore, the establishment of a suitable animal model is significant for the study, and improvement the diagnosis and treatment of this disease. MethodsTwenty four mice were divided into observation group (n=12)and control group (n=12). Mice in the observation group were feeding with methionine-choline-deficient diet (MCDD)for 3 weeks, the control group feeding normally. To observe the general condition of the mice dynamicly, and regularly weighed; 4 mice were killed in observation group and contral group each time at 8th,15th, 22nd day; then collected the serum samples for detecting of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycerides (TG), liver specimens for pathological diagnosis and non-alcoholic fatty liver disease active score (NAS). ResultsMCDD induced mice were different with the control group in general condition and weight, pathological analysis defined that hepatic fatty had changed since 8th day, and the NAS scores were always between 3 and 4 at 8th day, 15th day, significant different with the 22nd day (t=2.601, P=0.04; t=3.57, P=0.019). The levels of ALT, AST were increasing with the time, and the average numbers have upward trends, but there is no statistically significant differences. The level of TG was increasing also, and had statistically significant differences with control group in all the time. The level of ALT was positively correlated with the pathological score,but the correlation was not strong (r=0.574, P=0.032). ConclusionTwo weeks of MCDD induced stable NAFL animal model, with significant general condition change, loss of weight, increase of TG.
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