任双双,邓玉.p-STAT3/p-AKT信号通路及其靶基因CyclinD1在基底细胞癌皮损中的表达及其临床意义[J].转化医学杂志,2020,9(6):345-350
p-STAT3/p-AKT信号通路及其靶基因CyclinD1在基底细胞癌皮损中的表达及其临床意义
Clinical significance of p-STAT3/p-AKT signaling pathway and its target gene CyclinD1in skin lesions of basal cell carcinoma
  
DOI:
中文关键词:  基底细胞癌皮损  磷酸化转录信号转导子及激活子3/磷酸化蛋白激酶B信号通路  细胞周期素D1
英文关键词:Basal cell carcinoma skin lesion  Phosphorylated signal transducer and activator of transcription-3/phosphorylated protein kinase B signaling pathway (p-STAT3/P-AKT)  Cyclin D1
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作者单位
任双双 山东省济南市人民医院病理科 
邓玉 山东省济南市人民医院病理科 
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中文摘要:
      目的分析磷酸化转录信号转导子及激活子3(phosphorylated signal transducer and activator of transcription-3,p-STAT3)/磷酸化蛋白激酶B(phosphorylated protein kinase B,p-AKT)信号通路及其靶基因细胞周期素D1(cyclinD1)在基底细胞癌(basal cell carcinoma,BCC)皮损中的表达及其临床意义。方法选取2010年1月—2019年8月在山东省济南市人民医院皮肤科就诊且经组织病理及免疫组化确诊为BCC的60例患者石蜡标本,以及在本院接受外科整形手术并留存的正常皮肤组织标本的患者,分别纳入BBC组及正常组。分别采用免疫组化法、PCR法、Western印迹实验检测皮损处p-STAT3、p-AKT及cyclinD1表达,并分析BCC患者皮损处p-STAT3 mRNA、p-AKT mRNA、cyclinD1 mRNA与临床病理的关系。结果BCC组p-STAT3、p-AKT、cyclinD1平均光密度值,p-STAT3 mRNA、p-AKT mRNA、cyclinD1 mRNA及p-STAT3蛋白、p-AKT蛋白、cyclinD1蛋白表达显著高于正常组(P<0.05);且IBCC型BCC患者皮损中p-STAT3 mRNA、p-AKT mRNA、cyclinD1 mRNA表达显著高于NIBCC患者(P<0.05);BCC皮损中p-STAT3 mRNA、p-AKT mRNA、cyclinD1 mRNA表达与病理类型有显著相关性(P<0.01)。结论BCC皮损处存在p-STAT3、p-AKT、cyclinD1高表达现象,且IBCC型BCC患者皮损处p-STAT3 mRNA、p-AKT mRNA、cyclinD1 mRNA表达更高,值得临床重视。
英文摘要:
      ObjectiveTo analyze the clinical significance of phosphorylated signal transducer and activator of transcription-3 (p-STAT3)/phosphorylated protein kinase B (p-AKT) signaling pathway and its target gene cyclin D1 in skin lesions of basal cell carcinoma (BCC). MethodsThe BCC paraffin standard was derived from BCC patients diagnosed by histopathology and immunohistochemistry who visited the Department of Dermatology in our hospital from January 2010 to August 2019. The normal skin tissue samples were obtained from patients who underwent plastic surgery in our hospital during the same period and retained some excess skin tissue, which were included in the BBC group and the normal group respectively. The expressions of P-STAT3, P-AKT, and cyclinD1 in skin lesions were detected by immunohistochemistry, PCR, and Western blot, respectively, and the relationship between p-STAT3 mRNA, P-AKT mRNA, and cyclinD1 mRNA in skin lesions of BCC patients and clinicopathology was analyzed. ResultsThe average optical density values of P-STAT3, P-AKT, and cyclinD1 in the BCC group were significantly higher than those in the normal group (P<0.05). Moreover, the expressions of p-STAT3 mRNA, P-AKT mRNA and cyclinD1 mRNA in the skin lesions of IBCC patients were significantly higher than those of NIBCC patients (P<0.05). The expressions of P-STAT3 mRNA, P-AKT mRNA and cyclinD1 mRNA in BCC lesions were significantly correlated with the pathological type (P<0.01). ConclusionThere is high expression of p-STAT3, p-AKT and cyclinD1 in BCC skin lesions, and the expression of p-STAT3 mRNA, p-AKT mRNA and cyclinD1 mRNA is higher in skin lesions of IBCC type BCC, which deserves clinical attention.
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