| 智鹏,杨波,陈浩然,陈熙勐,迟小华,张钧栋,郭斌,李卓阳,刘格良,张皓旻,王毅兴,卢学春.α干扰素抗病毒相关全基因组表达谱的生物信息学分析及其对新型冠状病毒肺炎潜在药物研究的意义[J].转化医学杂志,2020,9(6):368-372 |
| α干扰素抗病毒相关全基因组表达谱的生物信息学分析及其对新型冠状病毒肺炎潜在药物研究的意义 |
| Bioinformatics analysis of the whole genome expression profile of alpha interferon antiviral correlation and its significance for the potential drug research of novel coronavirus pneumonia |
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| DOI： |
| 中文关键词: α干扰素 新型冠状病毒肺炎 生物信息学 基因表达谱 |
| 英文关键词:α-interferon COVID-19 Bioinformatics Gene expression profile |
| 基金项目:2017年度国家老年疾病临床医学研究中心招标课题(NCRCG-PLAGH-2017011)；中国人民解放军总医院转化医学项目(2017TM-020)；上海浦东新区卫健委学科带头人计划（PWRd2019-04）；上海浦东新区中医治未病高峰学科（PDZY-2018-0603）；2019年度保健专项科研课题（19BJZ28）;军队保健人群老年共病的新型危险因素预警及干预研究(18BJZ32) |
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| 中文摘要: |
| 目的通过分析α干扰素抗病毒相关全基因组表达谱，探索其对新型冠状病毒肺炎的潜在治疗意义。方法应用R语言对来自基因表达数据库(gene expression omnibus，GEO)的α干扰素相关的全基因组表达谱开展差异分析、富集分析、蛋白互作分析，再应用自主研发的表观精准治疗预测平台（epigenomic precision medicine prediction platform，EpiMed），寻找与α干扰素调控基因表达谱呈负相关的疾病类型，以及呈正相关的药物。结果α干扰素对基因组表达谱影响复杂，其调控的核心基因有OAS1、MX1、OASL、ISG15、IST1、IRF7等，参与病毒生命周期的负调控、B细胞受体信号通路、肝炎、双链RNA绑定、人类免疫缺陷病毒感染，以及JAK-STAT信号通路等。进一步分析，α干扰素调控的基因组表达谱与社区获得性肺炎和脓毒症等感染性疾病呈高度负相关，而与利托那韦、利巴韦林、奈韦拉平、氟伐他汀呈高度正相关。结论基于α干扰素抗病毒相关基因组表达谱的分析对于探索新型冠状病毒肺炎潜在治疗药物具有一定的借鉴意义。 |
| 英文摘要: |
| ObjectiveExplore the potential therapeutic significance of α-interferon antiviral-related genome-wide expression profiles for coronavirus disease 2019. MethodsApplication of R language to differential analysis, enrichment analysis, and protein interaction analysis of interferon-alpha-related whole-genome expression profiles from the gene expression omnlbus (GEO). Applied epigenomic precision medicine prediction platform (EpiMed), to explore disease types that are negatively related to alpha interferon-regulated gene expression profiles, and drugs that are positively related. ResultsThe effects of interferon-alpha on the transcriptome are complex. The effects of core genes OAS1, MX1, OASL, ISG15, IST1, IRF7,etc. include negative regulation of the viral life cycle, B cell receptor signaling pathway, hepatitis, double-stranded RNA binding, human immunodeficiency virus infection. And related pathways such as the JAK-STAT signaling pathway. At the same time, the transcriptome tag of interferon-alpha is highly negatively related to infectious diseases such ascommunity-acquired pneumonia and sepsis, and highly positively related to the guidelines published by the drug Ritonavir、Ribavirin、Nevirapine and Fluvastatin. ConclusionThe analysis of the expression profile of the anti-virus related genomes of α-interferon has certain reference significance for exploring new potential therapeutic drugs for coronavirus pneumonia (NCP）. |
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