张宁坤,高连如,赵力,王丽华,朱莹,刘秋颖,刘玲,苗晨,郝璐,陈宇.小鼠心肌梗死模型的快速制作方法研究[J].转化医学杂志,2020,9(6):373-377
小鼠心肌梗死模型的快速制作方法研究
Study on the rapid establishment of myocardial infarction model in mice
  
DOI:
中文关键词:  小鼠  心肌梗死  模型
英文关键词:Mice  Myocardial infarction  Model
基金项目:国家自然科学基金(81370238);中国人民解放军总医院医疗大数据与人工智能研发项目(2019MBD-057)
作者单位
张宁坤 中国人民解放军总医院第六医学中心心内科 
高连如 中国人民解放军总医院第六医学中心心内科 
赵力 中国人民解放军总医院第六医学中心心内科 
王丽华 中国人民解放军总医院第六医学中心心内科 
朱莹 中国人民解放军总医院第六医学中心心内科 
刘秋颖 中国人民解放军总医院第六医学中心超声诊断科 
刘玲 中国人民解放军总医院第六医学中心心内科 
苗晨 中国人民解放军总医院第六医学中心心内科 
郝璐 中国人民解放军总医院第六医学中心心内科 
陈宇 中国人民解放军总医院第六医学中心心内科 
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中文摘要:
      目的探讨小鼠心肌梗死模型的快速建立方法及成功率。方法小鼠诱导麻醉后,经呼吸面罩持续麻醉,于胸骨左缘第3~4肋间分离胸大肌。通过挤压法将心脏挤出胸腔,结扎前降支,将心脏放回胸腔,胸大肌覆盖复位。观察制作小鼠心肌梗死模型时间,心肌梗死成功率、死亡率,术后检查心电图,超声检查左室射血分数(left ventricular ejection fraction,LVEF)、左室收缩末内径(left ventricular end-systolic diameter,LVESD)、左室舒张末内径(left ventricular end-diastolic diameter,LVEDD)、左室短轴缩短率(left ventricular fractional shortening,LVFS)及左室前壁(left ventricular anterior wall,LVAW)厚度较术前变化情况,术后2周处死小鼠,观察心肌梗死情况及HE染色心肌组织变化。结果制作小鼠心肌梗死模型时间是(40±10)s,心肌梗死模型成功率94%,小鼠存活率96%,心电图显示小鼠术后心电图ST段显著抬高;模型制作后超声检查可见术后左室舒缩功能明显减弱,LVEF较术前降低[(27.5±3.9)%,P<0.01],LVESD较术前增加[(1.07±0.23)mm,P<0.01],LVEDD较术前增加[(1.41±0.29)mm,P<0.01],LVFS较术前降低[(8.1±3.1)%,P<0.05],LVAW较术前降低[(0.44±0.06)mm,P<0.01];术后取小鼠心脏LVAW冠状面切开可见厚度明显低于正常心肌,病理检查HE染色可见大量疤痕组织形成。结论本研究探讨了新的制作小鼠心肌梗死模型方法,通过优化操作流程,熟练掌握操作技巧,克服了现有技术的弊端,本方法在无气管插管支持呼吸的情况下可快速制作心肌梗死模型,成功率高,死亡率低,在涉及小鼠心肌梗死模型的研究中可以极大提高实验效率。
英文摘要:
      ObjectiveTo investigate the rapid establishment of mouse myocardial infarction model and its success rate. MethodsMice after induction of anesthesia with the breathing mask, on the left breast separation between 3 and 4 ribs pectoralis major, by squeezing the heart out of the chest, the anterior descending ligation, the heart back into the chest, the pectoralis major cover reset, observe the production time of myocardial infarction model in mice, myocardial infarction, the success rate, mortality and postoperative ecg examination, inspection ultrasonic cardiac function left ventricular ejection fraction (LVEF), left ventricular end-systolic diameter (LVESD), left ventricular end-diastolic diameter (LVEDD), left ventricular fractional shortening (LVFS), left ventricular anterior wall (LVAW) compared with preoperative and postoperative death mice to observe the myocardial infarction 2 weeks and HE dyed myocardial tissue changes. ResultsThe time to make the mouse model of myocardial infarction was (40±10) seconds, the success rate of the model of myocardial infarction was 96%, the survival rate of the mouse was 94%, and the ecg showed that the ST segment of the ecg was significantly increased after the operation. After the model was made, ultrasound examination showed that the postoperative left ventricular systolic reduction was significantly reduced, LVEF decreased by [(27.5±3.9)%, P<0.01], LVESD increased by [(1.07±0.23)mm, P<0.01], LVEDD increased by [(1.41±0.29)mm, P<0.01], LVFS decreased by [(8.1±3.1)%, P<0.05], LVAW decreased by [(0.44±0.06)mm, P<0.01]. The thickness of the coronal plane of the anterior wall of the left ventricle of the mouse heart was significantly lower than that of the normal myocardium after the operation. ConclusionThis method can be used to make mouse myocardial infarction model, optimize the operation process and master the operation skills, and overcome the disadvantages of the existing technology. In the absence of endotracheal intubation to support respiration, the myocardial infarction model can be quickly made, with a high success rate and a low mortality rate. The experimental efficiency can be greatly improved in the research involving the mouse myocardial infarction model.
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