刘 静,牛亚楠,孙力超.CEP170在肝癌中的表达及对肝癌细胞增殖的影响[J].转化医学杂志,2021,10(5):275-280+286
CEP170在肝癌中的表达及对肝癌细胞增殖的影响
Analysis of CEP170 expression in liver cancer and its role in proliferation of liver cancer cells
  
DOI:
中文关键词:  肝癌  临床蛋白质组学肿瘤分析联合会  中心体蛋白170  细胞增殖  靶向治疗
英文关键词:Liver cancer  Clinical proteomic tumor analysis consortium(CPTAC)  Centrosomal protein 170(CEP170)  Cell proliferation  Targeted therapy
基金项目:国家自然科学基金(81773170)
作者单位
刘 静 国家癌症中心国家肿瘤临床医学研究中心中国医学科学院北京协和医学院肿瘤医院 
牛亚楠 国家癌症中心国家肿瘤临床医学研究中心中国医学科学院北京协和医学院肿瘤医院 
孙力超 国家癌症中心国家肿瘤临床医学研究中心中国医学科学院北京协和医学院肿瘤医院 
摘要点击次数: 169
全文下载次数: 156
中文摘要:
      目的 研究中心体蛋白170(centrosomal protein170,CEP170)在肝癌组织中的表达及其对肝癌细胞增殖能力的影响。方法 从临床蛋白质组学肿瘤分析联合会(clinical proteomic tumor analysis consortium,CPTAC )数据库下载肝癌蛋白质组学数据及临床信息,R包limma分析肿瘤组织与正常组织CEP170蛋白表达差异,UALCAN及cBioPortal在线分析TCGA数据库中CEP170转录水平及基因拷贝数变异。利用Kaplan-Meier法进行生存分析,ROC曲线分析检验预测效应。免疫组化(immunohistochemistry,IHC)验证肿瘤组织与正常组织表达差异。特异性siRNA敲降肝癌细胞株Huh-7 CEP170表达,CCK-8法检测细胞增殖能力,平板克隆实验观察细胞克隆形成能力差异,流式细胞仪分析细胞周期变化。结果 肝癌组织中CEP170蛋白及转录水平显著高于正常组织(P<0.001),CEP170高表达是患者预后不良的独立风险因素。基因组DNA拷贝数在6%的癌组织中存在扩增。IHC结果可见CEP170在肿瘤组织中表达异常升高。敲降肝癌细胞Huh-7的CEP170表达后,细胞增殖及克隆形成能力显著降低(P<0.001),细胞周期发生G0/G1期阻滞。结论 CEP170在肝癌组织中异常高表达,降低CEP170表达能够显著抑制人肝癌细胞Huh-7增殖且细胞周期发生G0/G1期阻滞,CEP170是肝癌治疗的潜在靶点。
英文摘要:
      Objective To study the expression of Centrosomal Protein 170 (CEP170) in liver cancer tissues and its effect on the proliferation of liver cancer cells. Methods The liver cancer proteomics data and clinical information were downloaded from CPTAC (clinical proteomic tumor analysis consortium) database. The limma R package was used to analyze the differently expressed genes between cancer and adjacent tissues. The UALCAN and cBioPortal online webserver were used to ayalyse the CEP170 transcriptome and gene copy number in TCGA database. Kaplan-Meier method was used for survival analysis, and receiver operating characteristic(ROC) curve analysis was used to test the predictive power. Immunohistochemistry (immunohistochemistry, IHC) was used to verify the difference of CEP170 expression level between the cancer and adjacent tissues. Specific siRNA was used to knocked down the expression level of CEP170 in liver cancer cell line Huh-7. CCK-8 method was used to detect cell proliferation ability, and plate cloning experiment was used to test the cell clone formation ability. Cell cycle change was analyzed by flow cytometry. Results The protein and transcription levels of CEP170 in the liver cancer tissues were significantly higher than that of in the adjacent tissues (P<0.001). The high expression of CEP170 was an independent risk factor for poor prognosis. Genomic DNA copy number was amplified in 6% of the cancer samples. IHC results showed that the expression of CEP170 was abnormally increased in cancer tissues. After knocking down the expression of CEP170 in hepatocarcinoma cell line Huh-7, the cell proliferation and clonal formation ability was significantly reduced (P<0.001), and the cell cycle was blocked in G0/G1 phase. Conclusion CEP170 is abnormally expressed in liver cancer tissues. Down-regulation of CEP170 inhibits the proliferation of Huh-7 human liver cancer cells and the G0/G1 phase block of the cell cycle. CEP170 is a potential target for the treatment of liver cancer.
查看全文  查看/发表评论  下载PDF阅读器
关闭